Course Syllabus
Time frame of the course
This online class will be held on 4 hrs per week. The total hours of the course are 100 hrs. The last 15-20 minutes of the session will be for live interaction and question answers. Study material will be provided by respective teachers. Detailed session wise plan will be shared with the registered students. Classes will be conducted online via google meet.
BS 01: Biomedical Instrumentation
Theory:
Unit-1
Biopotentials, Bioamplifiers and Bioelectrodes: Introduction to bio-electric potential, bio-amplifier, components of man Instrument system, Types of biomedical systems, Properties of electrodes.
Unit-2
Cardiac vascular system & measurements: ECG: origin, Instrumentation, Blood pressure measurements, Pacemakers- Internal, External, Oximeters.
Unit-3
Respiratory Measurement Systems: Types of volume, types of measurements, Instrumentation of respiratory system,
Unit-4
Nervous system: Action potential of brain, brain wave, Instrumentation of Electroencephalography (EEG), electrodes used for recording EEG analysis.
Practical:
Unit-1
Describe the method to record and analyse various bio-electric signals (e.g. ECG, EEG etc)
Unit-2
Demonstrate measurement of basic medical parameters such as blood pressure, pulse rate.
BS02: Analytical Instrumentation
Theory:
Unit-1
Molecular Spectroscopy: Methods of Analysis: Colorimetry and Spectrophotometry: Introduction, theory: molecular energy levels, types of molecular transitions, Lambert-Beer‟s Law and limitations, types of sources, monochromators and detectors, Instrumentation of single beam and double beam instrument.
Unit-2
Infrared Spectroscopy: Theory, diatomic molecules as a simple harmonic oscillator, instrumentation, sample handling techniques.
Unit-3
UV Spectroscopy: Principle, comparison of atomic and molecular spectroscopy, atomic transitions, atomic absorption,
Unit-4
Separation methods: Theory of chromatography; instrumentation and applications of Paper chromatography, Thin layer chromatography (TLC).
Column chromatography: Principle, process of elution through a column, chromatogram, band broadening, column efficiency, column resolution, Ion exchange chromatography and Gel exclusion chromatography.
Vertical and Horizontal gel electrophoresis: Methods and aaplication.
Unit-5
Gas Chromatography (GC): carrier gases, different type of injection systems, columns, stationary phases and detectorsHigh Performance Liquid Chromatography (HPLC): mobile phase, isocratic and gradient elution, pumps, injection systems, columns, stationary phases, normal phase and reverse phase chromatography, detectors and their application.
Unit-6
Flow cytometer: Sample preparation and application.
Unit-7
Electron Microscopy: SEM and TEM; Sample preparation and application.
Unit-8
Compound microscopic techniques; Different staining techniques and their application.
Unit-9
PCR: Methods and application
Unit-10
Blotting techniques: Methods and applications.
Practical:
Unit-1
Examining the properties of sample tested on Colorimeter.
Unit-2
Examine and determine the properties and concentration of a given sample using Colorimeter and UV-VIS spectrophotometer
Unit-3
Carry out comparative analysis of given sample with standard sample using spectroscopic and chromatographic instruments
Unit-4
Thin layer chromatographic (TLC) separation of samples from different origin (Biological / Pharmaceutical / Food).
Unit-5
Demonstration on qualitative analysis of samples using Gas chromatography and qualitative analysis of samples using High Performance Liquid Chromatography
Unit-6
Demonstration of sample preparation in electron microscope.
Unit-7
DNA amplification by PCR technique.
Unit-8
DNA and Protein separation by electrophoresis.